human igf 1 elisa kit  (Cusabio)

Journal: Journal of Dental Sciences

Article Title: Lunar life drives jawbone formation

doi: 10.1016/j.jds.2025.03.032

Figure Lengend Snippet: Figure 3 Effect of partial gravity on the mandibular bone. (A) Histological findings and gene expression of insulin-like growth factor 1 (IGF-1) between 1/6G and 1G. The intensities were categorized into 4 groups (0e3) and randomly selected some fields. Scale bar Z 100 mm. (B) Histological findings of type I collagen at 1/6G and 1G. Scale bar Z 100 mm. (C) Quantification of mandibular bone using CT images between 1/ 6G and 1G. Scale bar Z 2 mm. Yellow box: measuring area; red dotted line: cutting plane of the images. *P < 0.05 and ***P < 0.001. ns, Not significant. TV: Tissue volume, BV: Bone volume, BS: Bone surface, BV/TV: Bone volume fraction.

Article Snippet: Anti-dentin matrix protein 1 (DMP1) (Invitrogen, PA5-103323, Carlsbad, CA, USA), secreted phosphoprotein 1 (SPP1) (Invitrogen, PA5-141,129), runtrelated transcription factor 2 (RUNX2) (Abcam, ab192256, Cambridge, UK), bone morphogenetic protein 2 (BMP2) (Bioss, bs-1012R, Boston, MA, USA), Sp7 (Abcam, ab209484), cathepsin K (Abcam, ab300569), and insulin-like growth factor 1 (IGF-1) (Proteintech, 28530-1-AP, Rosemont, IL, USA) antibodies were used after dilution with primary antibody diluent (Leica).

Techniques: Gene Expression

Journal: Journal of Dental Sciences

Article Title: Lunar life drives jawbone formation

doi: 10.1016/j.jds.2025.03.032

Figure Lengend Snippet: Figure 5 Overview of the effect of lunar gravity on the jawbone. Bone formation was promoted, while resorption was not, under lunar gravity (1/6G), suggesting an association with mastication. DMP1: Dentin matrix acidic phosphoprotein 1, IGF-1: Insulin-like growth factor 1, SPP1: Secreted phospho- protein 1.

Article Snippet: Anti-dentin matrix protein 1 (DMP1) (Invitrogen, PA5-103323, Carlsbad, CA, USA), secreted phosphoprotein 1 (SPP1) (Invitrogen, PA5-141,129), runtrelated transcription factor 2 (RUNX2) (Abcam, ab192256, Cambridge, UK), bone morphogenetic protein 2 (BMP2) (Bioss, bs-1012R, Boston, MA, USA), Sp7 (Abcam, ab209484), cathepsin K (Abcam, ab300569), and insulin-like growth factor 1 (IGF-1) (Proteintech, 28530-1-AP, Rosemont, IL, USA) antibodies were used after dilution with primary antibody diluent (Leica).

Techniques: